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Photo Jan Forsman

Jan Forsman

Professor

Photo Jan Forsman

Molecular evidence of stereo-specific lactoferrin dimers in solution.

Author

  • Björn Persson
  • Mikael Lund
  • Jan Forsman
  • Dereck E W Chatterton
  • Torbjörn Åkesson

Summary, in English

Gathering experimental evidence suggests that bovine as well as human lactoferrin self-associate in aqueous solution. Still, a molecular level explanation is unavailable. Using force field based molecular modeling of the protein-protein interaction free energy we demonstrate (1) that lactoferrin forms highly stereo-specific dimers at neutral pH and (2) that the self-association is driven by a high charge complementarity across the contact surface of the proteins. Our theoretical predictions of dimer formation are verified by electrophoretic mobility and N-terminal sequence analysis on bovine lactoferrin.

Department/s

  • Computational Chemistry
  • eSSENCE: The e-Science Collaboration

Publishing year

2010

Language

English

Pages

187-189

Publication/Series

Biophysical Chemistry

Volume

151

Issue

3

Document type

Journal article

Publisher

Elsevier

Topic

  • Theoretical Chemistry (including Computational Chemistry)

Status

Published

ISBN/ISSN/Other

  • ISSN: 1873-4200