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Photo Marie Skepö

Marie Skepö

Professor

Photo Marie Skepö

Spontaneous Formation of Cushioned Model Membranes Promoted by an Intrinsically Disordered Protein

Author

  • Yuri Gerelli
  • Amanda Eriksson Skog
  • Stephanie Jephthah
  • Rebecca J.L. Welbourn
  • Alexey Klechikov
  • Marie Skepö

Summary, in English

In this article, it is shown that by exposing commonly used lipids for biomembrane mimicking studies, to a solution containing the histidine-rich intrinsically disordered protein histatin 5, a protein cushion spontaneously forms underneath the bilayer. The underlying mechanism is attributed to have an electrostatic origin, and it is hypothesized that the observed behavior is due to proton charge fluctuations promoting attractive electrostatic interactions between the positively charged proteins and the anionic surfaces, with concomitant counterion release. Hence, we anticipate that this novel "green" approach of forming cushioned bilayers can be an important tool to mimic the cell membrane without the disturbance of the solid substrate, thereby achieving a further understanding of protein-cell interactions.

Department/s

  • Computational Chemistry
  • LINXS - Institute of advanced Neutron and X-ray Science
  • eSSENCE: The e-Science Collaboration

Publishing year

2020

Language

English

Pages

3997-4004

Publication/Series

Langmuir

Volume

36

Issue

15

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Biological Sciences

Status

Published

ISBN/ISSN/Other

  • ISSN: 0743-7463